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Proteintech rab8a
Rab8a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 40 article reviews

rab8a - by Bioz Stars, 2026-05

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HnRPD is identified to be an RAB8A mRNA-interacting protein. A The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; error bar, SD. N = 3. B qRT-PCR analysis of co-precipitated RAB8A mRNA by GPR137 antibody in the RIP assay in SK-OV-3 cells. IgG was used as a negative control. N = 3. C qRT-PCR analysis of co-precipitated RAB8A mRNA by GPR137 antibody in the RIP assay in A2780 cells. IgG was used as a negative control. N = 3. D An experimental model showing the procedure of IP and RNA pull-down with LC-MS/MS analysis. E LC-MS/MS analysis showing the identified proteins with difference in abundance from GPR137 antibody-IP group versus the corresponding control. F LC-MS/MS analysis showing the identified proteins with difference in abundance from RAB8A RNA pull-down group versus the corresponding control

Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: HnRPD is identified to be an RAB8A mRNA-interacting protein. A The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; error bar, SD. N = 3. B qRT-PCR analysis of co-precipitated RAB8A mRNA by GPR137 antibody in the RIP assay in SK-OV-3 cells. IgG was used as a negative control. N = 3. C qRT-PCR analysis of co-precipitated RAB8A mRNA by GPR137 antibody in the RIP assay in A2780 cells. IgG was used as a negative control. N = 3. D An experimental model showing the procedure of IP and RNA pull-down with LC-MS/MS analysis. E LC-MS/MS analysis showing the identified proteins with difference in abundance from GPR137 antibody-IP group versus the corresponding control. F LC-MS/MS analysis showing the identified proteins with difference in abundance from RAB8A RNA pull-down group versus the corresponding control

Article Snippet: GPR137 (bs-16270R, 1:1000) antibody and RAB8A (bs-6176R, 1:1000) antibody were acquired from Bioss Biotechnology (Beijing, China), while Flag-tag (M185-3, 1:10000) antibody was obtained from MBL Biotechnology (Beijing, China).

Techniques: Transfection, Plasmid Preparation, Expressing, Control, Cell Culture, Quantitative RT-PCR, Negative Control, Liquid Chromatography with Mass Spectroscopy

Molecular docking analysis of GPR137-hnRPD and hnRPD- RAB8A RNA. A Docking results by HDOCK server. Yellow indicates GPR137, purple indicates hnRPD. B Docking results visualized in the form of Surface. Red indicates protein-protein contact area. C Interaction of GPR137-hnRPD analyzed by PLIP. D Docking results by HDOCK server. Green indicates RAB8A RNA, purple indicates hnRPD. E Docking results visualized in the form of Surface. Red indicates protein-RNA contact area. F Interaction of hnRPD-RAB8A RNA analyzed by PLIP

Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: Molecular docking analysis of GPR137-hnRPD and hnRPD- RAB8A RNA. A Docking results by HDOCK server. Yellow indicates GPR137, purple indicates hnRPD. B Docking results visualized in the form of Surface. Red indicates protein-protein contact area. C Interaction of GPR137-hnRPD analyzed by PLIP. D Docking results by HDOCK server. Green indicates RAB8A RNA, purple indicates hnRPD. E Docking results visualized in the form of Surface. Red indicates protein-RNA contact area. F Interaction of hnRPD-RAB8A RNA analyzed by PLIP

Techniques:

GPR137 promotes RAB8A mRNA stability through elevating hnRPD expression. A Co-IP of endogenous GPR137 and hnRPD in SK-OV-3 cells. IP: GPR137, WB: hnRPD. IgG was used as a negative control. B Co-IP of endogenous hnRPD and GPR137 in A2780 cells. IP: hnRPD, WB: GPR137. IgG was used as a negative control. C qRT-PCR analysis of co-precipitated RAB8A mRNA by hnRPD antibody in the RIP assay in SK-OV-3 cells. IgG was used as a negative control. ** p < 0.01 versus IgG; error bar, SD. N = 3. D RNA pull-down assay in A2780 cells with RAB8A RNA or control RNA fragments. Protein abundance was normalized to GAPDH. E The mRNA expression of hnRPD in SK-OV-3 cells transfected with GPR137 shRNA or scrambled shRNA (shRNA-Con). Error bar, SD. N = 3. F The mRNA expression of hnRPD in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con). Error bar, SD. N = 3. G The protein expression of GPR137 and hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and cultured for 48 h. H The protein expression of GPR137 and hnRPD in A2780 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -). I The protein expression of Flag and hnRPD in OVCAR-8 cells transfected with a vector expressing Flag-GPR137 (Flag-GPR137, +) or a control Flag-tagged empty vector (Flag-GPR137, -). J Western blot analysis for GPR137 and hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and treated for different time periods with CHX. K Western blot analysis for Flag, GPR137 and hnRPD in OVCAR-8 cells transfected with a vector expressing Flag-GPR137 (Flag-GPR137, +) or a control Flag-tagged empty vector (Flag-GPR137, -) and treated for different time periods with CHX. L Western blot analysis for hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and treated with or without MG132. M The mRNA expression of RAB8A in SK-OV-3 cells transfected with hnRPD shRNA or scrambled shRNA (shRNA-Con). ** p < 0.01 versus shRNA-Con; error bar, SD. N = 3. N The mRNA expression of RAB8A in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con). ** p < 0.01 versus Con; error bar, SD. N = 3. O The mRNA levels of RAB8A in SK-OV-3 cells transfected with hnRPD shRNA (sh hnRPD) or scrambled shRNA (shCon) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus shCon; error bar, SD. N = 3. P The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; error bar, SD. N = 3. Q The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con) in combination with hnRPD shRNA (sh hnRPD) or scrambled shRNA and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; # p < 0.05 versus GPR137; error bar, SD. N = 3. R GEPIA database displayed the correlation between RAB8A and hnRPD

Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: GPR137 promotes RAB8A mRNA stability through elevating hnRPD expression. A Co-IP of endogenous GPR137 and hnRPD in SK-OV-3 cells. IP: GPR137, WB: hnRPD. IgG was used as a negative control. B Co-IP of endogenous hnRPD and GPR137 in A2780 cells. IP: hnRPD, WB: GPR137. IgG was used as a negative control. C qRT-PCR analysis of co-precipitated RAB8A mRNA by hnRPD antibody in the RIP assay in SK-OV-3 cells. IgG was used as a negative control. ** p < 0.01 versus IgG; error bar, SD. N = 3. D RNA pull-down assay in A2780 cells with RAB8A RNA or control RNA fragments. Protein abundance was normalized to GAPDH. E The mRNA expression of hnRPD in SK-OV-3 cells transfected with GPR137 shRNA or scrambled shRNA (shRNA-Con). Error bar, SD. N = 3. F The mRNA expression of hnRPD in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con). Error bar, SD. N = 3. G The protein expression of GPR137 and hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and cultured for 48 h. H The protein expression of GPR137 and hnRPD in A2780 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -). I The protein expression of Flag and hnRPD in OVCAR-8 cells transfected with a vector expressing Flag-GPR137 (Flag-GPR137, +) or a control Flag-tagged empty vector (Flag-GPR137, -). J Western blot analysis for GPR137 and hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and treated for different time periods with CHX. K Western blot analysis for Flag, GPR137 and hnRPD in OVCAR-8 cells transfected with a vector expressing Flag-GPR137 (Flag-GPR137, +) or a control Flag-tagged empty vector (Flag-GPR137, -) and treated for different time periods with CHX. L Western blot analysis for hnRPD in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137, +) or scrambled shRNA (shGPR137, -) and treated with or without MG132. M The mRNA expression of RAB8A in SK-OV-3 cells transfected with hnRPD shRNA or scrambled shRNA (shRNA-Con). ** p < 0.01 versus shRNA-Con; error bar, SD. N = 3. N The mRNA expression of RAB8A in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con). ** p < 0.01 versus Con; error bar, SD. N = 3. O The mRNA levels of RAB8A in SK-OV-3 cells transfected with hnRPD shRNA (sh hnRPD) or scrambled shRNA (shCon) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus shCon; error bar, SD. N = 3. P The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; error bar, SD. N = 3. Q The mRNA levels of RAB8A in OVCAR-8 cells transfected with a vector expressing GPR137 or a control empty vector (Con) in combination with hnRPD shRNA (sh hnRPD) or scrambled shRNA and cultured with actinomycin D (AcD) for the indicated time periods. * p < 0.05; ** p < 0.01 versus Con; # p < 0.05 versus GPR137; error bar, SD. N = 3. R GEPIA database displayed the correlation between RAB8A and hnRPD

Techniques: Expressing, Co-Immunoprecipitation Assay, Negative Control, Quantitative RT-PCR, Pull Down Assay, Control, Quantitative Proteomics, Transfection, shRNA, Plasmid Preparation, Cell Culture, Western Blot

GPR137-hnRPD-RAB8A axis regulates OC cell biological behaviors. A CCK-8 assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) and cultured for the indicated time periods. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. N = 3. B Wound healing assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) at 24 h. Migrated distance was statistically analyzed. Bar, 100 μm. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. C CCK-8 assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) and cultured for the indicated time periods. * p < 0.05 versus Control, # p < 0.05 versus hnRPD, ** p < 0.01 versus Control, ## p < 0.01 versus hnRPD; error bar, SD. N = 3. D Wound healing assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) at 24 h. Migrated distance was statistically analyzed. Bar, 100 μm. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. E - H Spearman correlation analysis plots showing the correlation between the pathway scores and the expression of gene hnRPD . In the plots, the x-axis represents the distribution of the expression of hnRPD , and the y-axis represents the distribution of the corresponding pathway score. The values at the top represent the results of the Spearman correlation analysis, including the p -value, and correlation coefficient. I Matrigel invasion assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) at 24 h. Bar, 100 μm. J Colony formation assays of OVCAR-8 cells infected with lentiviruses expressing GPR137 or control lentiviruses (in Control group) in combination with lentiviruses carrying hnRPD shRNA or scrambled shRNA (in Control group). K Quantitative analysis of (E). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. L Quantitative analysis of (F). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. M Matrigel invasion assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) at 24 h. Bar, 100 μm. N Colony formation assays of OVCAR-8 cells infected with lentiviruses expressing hnRPD or control lentiviruses (in Control group) in combination with lentiviruses carrying RAB8A shRNA or scrambled shRNA (in Control group). O Quantitative analysis of (I). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. P Quantitative analysis of (J). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. Q CCK-8 assays of SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA in combination with a hnRPD-expressing vector or an empty vector (Control) and cultured for the indicated time periods. * p < 0.05 versus Control, # p < 0.05 versus shGPR137; ** p < 0.01 versus Control, ## p < 0.01 versus shGPR137; error bar, SD. R Migrated distance was statistically analyzed in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA in combination with a hnRPD-expressing vector or an empty vector (Control) at 24 h. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-GPR137 + Control; error bar, SD. S Matrigel invasion assays of SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA (in Control group) in combination with a hnRPD-expressing vector or an empty vector (in Control group) at 24 h. Bar, 100 μm. T Colony formation assays of SK-OV-3 cells infected with lentiviruses carrying GPR137 shRNA or scrambled shRNA (in Control group) in combination with lentiviruses expressing hnRPD or control lentiviruses (in Control group). U Quantitative analysis of (O). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-GPR137 + Control; error bar, SD. N = 3. V Quantitative analysis of (P). * p < 0.05 versus shRNA-Con+Control, # p < 0.05 versus shRNA-GPR137 + Control; error bar, SD. N = 3

Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: GPR137-hnRPD-RAB8A axis regulates OC cell biological behaviors. A CCK-8 assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) and cultured for the indicated time periods. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. N = 3. B Wound healing assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) at 24 h. Migrated distance was statistically analyzed. Bar, 100 μm. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. C CCK-8 assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) and cultured for the indicated time periods. * p < 0.05 versus Control, # p < 0.05 versus hnRPD, ** p < 0.01 versus Control, ## p < 0.01 versus hnRPD; error bar, SD. N = 3. D Wound healing assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) at 24 h. Migrated distance was statistically analyzed. Bar, 100 μm. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. E - H Spearman correlation analysis plots showing the correlation between the pathway scores and the expression of gene hnRPD . In the plots, the x-axis represents the distribution of the expression of hnRPD , and the y-axis represents the distribution of the corresponding pathway score. The values at the top represent the results of the Spearman correlation analysis, including the p -value, and correlation coefficient. I Matrigel invasion assays of OVCAR-8 cells transfected with a GPR137-expressing vector or an empty vector (in Control group) in combination with hnRPD shRNA (shhnRPD) or scrambled shRNA (in Control group) at 24 h. Bar, 100 μm. J Colony formation assays of OVCAR-8 cells infected with lentiviruses expressing GPR137 or control lentiviruses (in Control group) in combination with lentiviruses carrying hnRPD shRNA or scrambled shRNA (in Control group). K Quantitative analysis of (E). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. L Quantitative analysis of (F). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N = 3. M Matrigel invasion assays of OVCAR-8 cells transfected with a hnRPD-expressing vector or an empty vector (in Control group) in combination with RAB8A shRNA (shRAB8A) or scrambled shRNA (in Control group) at 24 h. Bar, 100 μm. N Colony formation assays of OVCAR-8 cells infected with lentiviruses expressing hnRPD or control lentiviruses (in Control group) in combination with lentiviruses carrying RAB8A shRNA or scrambled shRNA (in Control group). O Quantitative analysis of (I). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. P Quantitative analysis of (J). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. Q CCK-8 assays of SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA in combination with a hnRPD-expressing vector or an empty vector (Control) and cultured for the indicated time periods. * p < 0.05 versus Control, # p < 0.05 versus shGPR137; ** p < 0.01 versus Control, ## p < 0.01 versus shGPR137; error bar, SD. R Migrated distance was statistically analyzed in SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA in combination with a hnRPD-expressing vector or an empty vector (Control) at 24 h. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-GPR137 + Control; error bar, SD. S Matrigel invasion assays of SK-OV-3 cells transfected with GPR137 shRNA (shGPR137) or scrambled shRNA (in Control group) in combination with a hnRPD-expressing vector or an empty vector (in Control group) at 24 h. Bar, 100 μm. T Colony formation assays of SK-OV-3 cells infected with lentiviruses carrying GPR137 shRNA or scrambled shRNA (in Control group) in combination with lentiviruses expressing hnRPD or control lentiviruses (in Control group). U Quantitative analysis of (O). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-GPR137 + Control; error bar, SD. N = 3. V Quantitative analysis of (P). * p < 0.05 versus shRNA-Con+Control, # p < 0.05 versus shRNA-GPR137 + Control; error bar, SD. N = 3

Techniques: CCK-8 Assay, Transfection, Expressing, Plasmid Preparation, Control, shRNA, Cell Culture, Infection

Suppression of hnRPD expression inhibits OC progression in vivo. A Nude mice with xenografts derived from OVCAR-8 cells stably expressing hnRPD or control. N = 6. B Image showing the size of the tumor xenografts from the two groups in (A). C Weights of xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. D Tumor volumes of xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. E The mRNA levels of RAB8A in xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. F RAB8A staining of RAB8A in xenografts in (B). N = 6. Bar, 20 μm. G IHC score for RAB8A staining in (F). ** p < 0.01 versus Con. H Graphic illustration of the intraperitoneal injection of control or hnRPD stable-overexpressing OVCAR-8 cells. I The metastasis and spread of control or hnRPD stable-overexpressing OVCAR-8 cells were monitored by IVIS Imaging System. N = 5. J Nude mice with xenografts derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). N = 6. K Image showing the size of the tumor xenografts from three groups. L Tumor volume was measured on day 3, 6, 9, 12, 15, and 18, after OVCAR-8 cell injection. N = 6. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. M Weights of xenografts derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). N = 6. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N Body weight of mice was examined on day 3, 6, 9, 12, 15, and 18, after OVCAR-8 cell injection. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. O KI-67 staining and RAB8A staining for tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD) on day 18 post inoculation. In Control group, tumor tissues were derived from OVCAR-8 cells infected with control lentiviruses in combination with lentiviruses carrying a scrambled shRNA sequence. Bar, 20 μm. P Statistical analysis of KI-67 index (%) from (F). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. Q IHC score for RAB8A staining in (O). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137. R The mRNA levels of RAB8A in formed tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. S The protein levels of RAB8A in formed tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). In Control group, tumor tissues were derived from OVCAR-8 cells infected with control lentiviruses in combination with lentiviruses carrying a scrambled shRNA sequence (labeled as GPR137, -; shhnRPD, -). T qRT-PCR analysis of co-precipitated RAB8A mRNA by hnRPD antibody in the RIP assay in tumor tissues. IgG was used as a negative control. ** p < 0.01 versus IgG; error bar, SD. N = 3. U RNA pull-down assay in tumor tissues with RAB8A RNA or control RNA fragments. Protein abundance was normalized to a Tubulin

Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: Suppression of hnRPD expression inhibits OC progression in vivo. A Nude mice with xenografts derived from OVCAR-8 cells stably expressing hnRPD or control. N = 6. B Image showing the size of the tumor xenografts from the two groups in (A). C Weights of xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. D Tumor volumes of xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. E The mRNA levels of RAB8A in xenografts in (B). N = 6. ** p < 0.01 versus Con; error bar, SD. F RAB8A staining of RAB8A in xenografts in (B). N = 6. Bar, 20 μm. G IHC score for RAB8A staining in (F). ** p < 0.01 versus Con. H Graphic illustration of the intraperitoneal injection of control or hnRPD stable-overexpressing OVCAR-8 cells. I The metastasis and spread of control or hnRPD stable-overexpressing OVCAR-8 cells were monitored by IVIS Imaging System. N = 5. J Nude mice with xenografts derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). N = 6. K Image showing the size of the tumor xenografts from three groups. L Tumor volume was measured on day 3, 6, 9, 12, 15, and 18, after OVCAR-8 cell injection. N = 6. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. M Weights of xenografts derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). N = 6. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. N Body weight of mice was examined on day 3, 6, 9, 12, 15, and 18, after OVCAR-8 cell injection. ** p < 0.01 versus Control, ## p < 0.01 versus GPR137; error bar, SD. O KI-67 staining and RAB8A staining for tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD) on day 18 post inoculation. In Control group, tumor tissues were derived from OVCAR-8 cells infected with control lentiviruses in combination with lentiviruses carrying a scrambled shRNA sequence. Bar, 20 μm. P Statistical analysis of KI-67 index (%) from (F). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. Q IHC score for RAB8A staining in (O). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137. R The mRNA levels of RAB8A in formed tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+GPR137; error bar, SD. S The protein levels of RAB8A in formed tumor tissues derived from OVCAR-8 cells stably expressing GPR137 or in combination with hnRPD shRNA (shhnRPD). In Control group, tumor tissues were derived from OVCAR-8 cells infected with control lentiviruses in combination with lentiviruses carrying a scrambled shRNA sequence (labeled as GPR137, -; shhnRPD, -). T qRT-PCR analysis of co-precipitated RAB8A mRNA by hnRPD antibody in the RIP assay in tumor tissues. IgG was used as a negative control. ** p < 0.01 versus IgG; error bar, SD. N = 3. U RNA pull-down assay in tumor tissues with RAB8A RNA or control RNA fragments. Protein abundance was normalized to a Tubulin

Techniques: Expressing, In Vivo, Derivative Assay, Stable Transfection, Control, Staining, Injection, Imaging, shRNA, Infection, Sequencing, Labeling, Quantitative RT-PCR, Negative Control, Pull Down Assay, Quantitative Proteomics

A ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr and immuno-stained with GT335, anti-acetylated tubulin and anti-Arl13b antibodies. ≥90 cells per sample were analyzed in three independent experiments. Error bars, S.D. ***p ≤ 0.001, **** p ≤ 0.0001. B ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, and immuno-stained with antibodies against GT335, Arl13b, Myo-Va, Cep89, Rab8a, Rabin8, IFT88, Cep290 and RPGRIP1L. Scale bar, 1µm. C ) sgRNA control and TBC1D19 knockout cells were serum starved as in panels A and B and visualized by staining with GT335 and antibodies against CP110. Scale bar, 1µm. N ≥ 90 cells per sample were analyzed in three independent experiments. Error bars, S.D. D ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, and immunostained with antibodies against Rab34 and Arl13b. Scale bar, 1µm. E ) sgRNA control and TBC1D19 knockout cells were serum starved as in panels A-C and immunostained with antibodies against GT335 and Arl13b. Scale bar, 1µm. F ) Primary cilia in sgRNA control and TBC1D19 knockout cells were examined by transmission electron microscopy (TEM) after 24 hr of serum starvation. Images of TBC1D19 knockout cilia from two consecutive sections are shown.

Journal: bioRxiv

Article Title: The tubulin poly-glutamylase complex, TPGC, is required for phosphatidyl inositol homeostasis and cilium assembly and maintenance

doi: 10.1101/2025.03.03.641315

Figure Lengend Snippet: A ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr and immuno-stained with GT335, anti-acetylated tubulin and anti-Arl13b antibodies. ≥90 cells per sample were analyzed in three independent experiments. Error bars, S.D. ***p ≤ 0.001, **** p ≤ 0.0001. B ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, and immuno-stained with antibodies against GT335, Arl13b, Myo-Va, Cep89, Rab8a, Rabin8, IFT88, Cep290 and RPGRIP1L. Scale bar, 1µm. C ) sgRNA control and TBC1D19 knockout cells were serum starved as in panels A and B and visualized by staining with GT335 and antibodies against CP110. Scale bar, 1µm. N ≥ 90 cells per sample were analyzed in three independent experiments. Error bars, S.D. D ) sgRNA control and TBC1D19 knockout cells were serum starved for 24 hr, and immunostained with antibodies against Rab34 and Arl13b. Scale bar, 1µm. E ) sgRNA control and TBC1D19 knockout cells were serum starved as in panels A-C and immunostained with antibodies against GT335 and Arl13b. Scale bar, 1µm. F ) Primary cilia in sgRNA control and TBC1D19 knockout cells were examined by transmission electron microscopy (TEM) after 24 hr of serum starvation. Images of TBC1D19 knockout cilia from two consecutive sections are shown.

Article Snippet: Mouse anti-α-tubulin (1:10000 for WB 66031-1-Ig), rabbit anti-Arl13b (1:2000 for IF and 1:1000 for WB, 17711-1-AP), rabbit anti-Rab8a (1:50 for IF, 55296-1-AP), rabbit anti-IFT88 (1:500 for IF, 13967-1-AP), rabbit anti-INPP5E (1:1000 for WB and 1:1000 for IF, 17797-1-AP), mouse anti-β-actin (1:10000 for WB, 66009-1-Ig PT), mouse anti-GAPDH (1:10000 for WB, 60004-1-Ig), rabbit ant-CCP1 (1:1000 for WB, 14067-1-AP), Rabbit anti-TBC1D19 (1:1000 for WB, 21085-1-AP) and rabbit anti-Tulp3 (1:1000 for WB, 1:150 for IF, 13637-1-AP), RPGRIP1L (1:150 for IF, 55160-1-AP) were from Proteintech.

Techniques: Control, Knock-Out, Staining, Transmission Assay, Electron Microscopy

A ) Control (sgCTL) and TBC1D19 knockout cells were treated with non-targeting control or CCP1 siRNA for 48 hr and serum starved for 24 hr, and extracts were subjected to immunoblotting with the indicated antibodies. B ) TBC1D19 knockout cells were infected with lentivirus expressing Flag-map4m or Flag-map4m-TTLL1 for 72 hr, serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. C ) Control (sgCTL), TBC1D19 knockout cells, and TBC1D19 knockout cells stably expressing TTLL5-EYFP or TTLL6-EYFP were serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. D ) Control (sgCTL), TBC1D19 -/- , C11ORF49 -/- , and TTLL1 -/- cells were serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. E ) sgCTL, C11ORF49 -/- , and LRRC49 -/- cells were serum starved for 24 hr, immuno-stained with antibodies against acetylated tubulin and INPP5E. N ≥ 50 cilia were counted per sample in two independent experiments. Error bars, S.D. ns, not significant. F ) 293T cells were co-transfected with GFP or GFP-TBC1D19 with mCherry-Arl13b for 32 hr and serum starved for 16 hr. Lysates were subjected to immunoprecipitation with GFP-trap beads and immuno-blotted with antibodies against GFP and mCherry.

Journal: bioRxiv

Article Title: The tubulin poly-glutamylase complex, TPGC, is required for phosphatidyl inositol homeostasis and cilium assembly and maintenance

doi: 10.1101/2025.03.03.641315

Figure Lengend Snippet: A ) Control (sgCTL) and TBC1D19 knockout cells were treated with non-targeting control or CCP1 siRNA for 48 hr and serum starved for 24 hr, and extracts were subjected to immunoblotting with the indicated antibodies. B ) TBC1D19 knockout cells were infected with lentivirus expressing Flag-map4m or Flag-map4m-TTLL1 for 72 hr, serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. C ) Control (sgCTL), TBC1D19 knockout cells, and TBC1D19 knockout cells stably expressing TTLL5-EYFP or TTLL6-EYFP were serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. D ) Control (sgCTL), TBC1D19 -/- , C11ORF49 -/- , and TTLL1 -/- cells were serum starved for 24 hr and subjected to immunoblotting with the indicated antibodies. E ) sgCTL, C11ORF49 -/- , and LRRC49 -/- cells were serum starved for 24 hr, immuno-stained with antibodies against acetylated tubulin and INPP5E. N ≥ 50 cilia were counted per sample in two independent experiments. Error bars, S.D. ns, not significant. F ) 293T cells were co-transfected with GFP or GFP-TBC1D19 with mCherry-Arl13b for 32 hr and serum starved for 16 hr. Lysates were subjected to immunoprecipitation with GFP-trap beads and immuno-blotted with antibodies against GFP and mCherry.

Techniques: Control, Knock-Out, Western Blot, Infection, Expressing, Stable Transfection, Staining, Transfection, Immunoprecipitation

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